Potential of Soursop Leaf Extract (Annona Muricata L.) as an Antimicrobial Against Staphylococcus epidermidis

Authors

  • Aqil Fauziy Universitas Muslim Indonesia
  • Nurelly N. Waspodo Universitas Muslim Indonesia
  • Andi Alamanda Irwan Universitas Muslim Indonesia
  • Yani Sodiqah Universitas Muslim Indonesia
  • Dhian Karina Universitas Muslim Indonesia
  • Muhammad Wirawan Harahap Universitas Muslim Indonesia

DOI:

https://doi.org/10.31101/ijhst.v7i1.3966
Abstract views 204 times

Keywords:

Annona muricata L, activity test, Staphylococcus epidermidis

Abstract

Staphylococcus epidermidis is an opportunistic bacterium that can cause infections, particularly in individuals with weakened immune systems. The increasing resistance to antibiotics has encouraged the use of medicinal plants such as soursop leaves (Annona muricata L.), which contain antibacterial compounds. This study aims to evaluate the antibacterial effectiveness of soursop leaf extract against the growth of Staphylococcus epidermidis at concentrations of 50%, 75%, and 100%. The study specifically investigated the inhibition zones formed at each concentration (50%, 75%, and 100%) of Annona muricata L. extract against Staphylococcus epidermidis, and compared the antibacterial activity across these three concentrations. This research used a laboratory experimental design with the Kirby-Bauer disc diffusion method to assess the antibacterial activity. The extract was prepared using 96% ethanol and tested at the concentrations stated. Gentamicin 10 μg was used as a positive control, while sterile distilled water served as the negative control. The inhibition zones were observed after 24 hours of incubation at 37°C. The results showed that soursop leaf extract at 50% and 75% concentrations produced inhibition zones categorized as resistant. At 100% concentration, the inhibition zone ranges from resistant to intermediate. The comparison across all three concentrations indicates that the 100% concentration demonstrated notable antimicrobial activity against Staphylococcus epidermidis.

Downloads

Download data is not yet available.

References

Halid, M., & others. (2022). Uji Aktivitas Antibakteri Ekstrak Daun Kopasanda (Chromolaena odorata L.) Terhadap Bakteri Staphylococcus epidermidis. Pharmaceutical and Traditional Medicine, 6(1), 1–7.

Komalasari, M., Alkausar, R., & Retnaningsih, A. (2021). TEST THE INHIBITORY POWER OF SOURSOP LEAF EXTRACT (Annona Muricata L) AGAINST Escerichia Coli AND Staphylococcus Aureus BACTERIA BY DISC DIFFUSION METHOD UJI DAYA HAMBAT EKSTRAK DAUN SIRSAK (Annona muricata L) TERHADAP BAKTERI Escherichia Coli DAN Staphyl. Jurnal Analis Farmasi, 6(2), 73–78.

Kresnapati, I. N. B. A., & Sofya, S. W. (2023). Aktivitas Antimikroba Ekstrak Etanol Daun Sirsak (Annona muricata L) Terhadap Bakteri Gram Negatif Eschericia coli. Jurnal Ners, 7(1), 477–483. https://doi.org/10.31004/jn.v7i1.12996

Lestari, H. D., & Asri, M. T. (2021). Aktivitas Antibakteri Ekstrak Kulit Buah Kakao (Theobroma cacao L.) Terhadap Staphylococcus epidermidis. LenteraBio : Berkala Ilmiah Biologi, 10(3), 302–308. https://doi.org/10.26740/lenterabio.v10n3.p302-308

Made Ionnandha, L., & Andriana, A. (2023). UJI DAYA HAMBAT EKSTRAK DAUN SIRSAK (Annona muricata Linn) TERHADAP BAKTERI Escherichia coli SECARA IN VITRO Inhibition Test Of Extract From Soursop Leaf (Annona muricata Linn) Against Escherichia coli Bacteria. Nusantara Hasana Journal, 2(11), Page.

Martsiningsih, A., Suyana, S., Noviani, A., Rahmawati, U., Sujono, S., & Dwi Astuti, F. (2023). Pengaruh Waktu Inkubasi Terhadap Diameter Zona Hambat Antibiotik Pada Uji Sensitivitas Bakteri Klebsiella Pneumonia. Meditory : The Journal of Medical Laboratory, 11(1), 1–8. https://doi.org/10.33992/meditory.v11i1.2361

Meitania Utami, S., Andriati, R., & Hamdiah, S. (2022). STUDI LITERATUR AKTIVITAS ANTIBAKTERI EKSTRAK DAUN SIRSAK (Annona muricata L.) TERHADAP BERBAGAI SAMPEL BAKTERI. In PHRASE (Pharmaceutical Science) Journal (Vol. 2, Issue 1).

Nadira, Raudah, S., & Latifah. (2017). Pengaruh Ekstrak Daun Sirsak (Annona muricata L.) Terhadap Pertumbuhan Bakteri Escherichia coli ATCC 25922 DAN Staphylococcus aureus ATCC 25923. Jurnal Medika: Karya Ilmiah Kesehatan, 2(2), 1–9.

Pomputius, W. F., Kilgore, S. H., & Schlievert, P. M. (2023). Probable enterotoxin-associated toxic shock syndrome caused by Staphylococcus epidermidis. BMC Pediatrics, 23(1), 1–6. https://doi.org/10.1186/s12887-023-03914-5

Rahman, F. A., Haniastuti, T., & Utami, T. W. (2017). Skrining fitokimia dan aktivitas antibakteri ekstrak etanol daun sirsak (Annona muricata L.) pada Streptococcus mutans ATCC 35668. Majalah Kedokteran Gigi Indonesia, 3(1), 1. https://doi.org/10.22146/majkedgiind.11325

Rizky, V. A., Siregar, S., Krisdianilo, V., & Khadijah, S. (2024). Efektivitas Sediaan Gel Antiseptik Tangan Ekstrak Daun Sirsak Annona muricata Linn Sebagai Antibakteri Terhadap Staphylococcus epidermidis. Bioma : Jurnal Biologi Makassar, 9, 105–112.

Sagita, P. (2021). Pengaruh Pemberian Daun Sirsak (Annona muricata) Terhadap Penyakit Diabetes Melitus. Jurnal Medika Hutama, 3(01 Oktober), 1265–1272.

Skovdal, S. M., Jørgensen, N. P., & Meyer, R. L. (2022). JMM Profile: Staphylococcus epidermidis. Journal of Medical Microbiology, 71(10), 1–5. https://doi.org/10.1099/jmm.0.001597

Widyananda, G. A. D., Mahendra, A. N., & Jawe, I. M. (2021). Efek antibakteri ekstrak etanol daun sirsak (Annona muricata L.) muda dan tua terhadap Pseudomonas aeruginosa ATCC 9027. Intisari Sains Medis | Intisari Sains Medis, 12(1), 212–218. https://doi.org/10.15562/ism.v12i1.915

Zainab, S., Choesrina, R., & Hazar, S. (2024). Uji Aktivitas Antibakteri Ekstrak Etanol Kulit Buah Sirsak (Annona Muricata L.) Terhadap Staphylococcus Epidermidis. In Bandung Conference Series: Pharmacy, 4(1), 68–77. https://doi.org/https://doi.org/10.29313/bcsp.v4i1.11910

Downloads

Published

2025-07-29

How to Cite

Fauziy, A., Waspodo, N. N., Irwan, A. A., Sodiqah, Y., Karina, D., & Harahap, M. W. (2025). Potential of Soursop Leaf Extract (Annona Muricata L.) as an Antimicrobial Against Staphylococcus epidermidis. International Journal of Health Science and Technology, 7(1), 83–89. https://doi.org/10.31101/ijhst.v7i1.3966

Issue

Vol. 7 No. 1 (2025): Juli

Section

Original Research

License

Copyright (c) 2025 Aqil Fauziy, Nurelly N. Waspodo, Andi Alamanda Irwan, Yani Sodiqah, Dhian Karina, Muhammad Wirawan Harahap

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

International Journal of Health Science and Technology allows readers to read, download, copy, distribute, print, search, or link to its articles' full texts and allows readers to use them for any other lawful purpose. The journal allows the author(s) to hold the copyright without restrictions. Finally, the journal allows the author(s) to retain publishing rights without restrictions

  • Authors are allowed to archive their submitted article in an open access repository
  • Authors are allowed to archive the final published article in an open access repository with an acknowledgment of its initial publication in this journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 Generic License.