Kemampuan Metabolisme Asam Linoleat oleh Kandidat Probiotik Potensial Asal Feses Bayi Asli Indonesia

Nosa Septiana Anindita; Muslih Anwar Widodo; Tiyas Tono Taufik; Tutik Dwi Wahyuningsih

doi:10.31101/jhes.433

Authors

Nosa Septiana Anindita Universitas â€˜Aisyiyah Yogyakarta, Indonesia
Muslih Anwar Widodo BPTBA - LIPI Gunung Kidul, Indonesia
Tiyas Tono Taufik Universita Gadjah Mada, Indonesia
Tutik Dwi Wahyuningsih Universita Gadjah Mada, Indonesia

DOI:

https://doi.org/10.31101/jhes.433

Keywords:

infant faeces, linoleic acid, probiotics

Abstract

Six probiotic candidates were grown overnight at 37 Â°C in De Man Rogosa Sharpe(MRS) broth supplemented with 0.4 mg/mL of linoleic acid. The concentrations of the metabolite products in the culture media were determined using GC-MS after extraction and direct transesterification of the fatty acids. Linoleic acid was added to the sample as an internal standard. The result showed that Lactobacilluscasei strainAPwas able to convertfreelinoleicacidto conjugated linoleic acid in themedia more than 60% while five other potential probiotics such as Lactobacillus casei AF, Lactobacillus casei AG, Pediococcus acidilactici AA, Pediococcus acidilactici BE and Pediococcus acidilactici BK have abilities to convert free linoleic acid to palmitic acid, stearic acid and oleic acid with varying percentages.

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Author Biography

Nosa Septiana Anindita, Universitas â€˜Aisyiyah Yogyakarta

S1 Bioteknologi Universitas â€˜Aisyiyah Yogyakarta

References

Alonso, L., E.P. Cuesta and S.E.Gilliland. 2003. Production of free conjugated linoleic acid by Lactobacillus acidophilus and Lactobacillus casei of human intestinal origin. J. Dairy Sci., 86: 1941â€“1946.

Anindita, N.S., Widodo., T.T. Taufiq dan T.D. Wahyuningsih. 2016. Identifikasi 16S rRNA Lactobacillus casei Kandidat Probiotik Asal Feses Bayi Indonesia Melalui Pola Fingerprinting PCR-RFLP. Prosiding Simposium Nasional Teknologi Terapan (SNTT), 4: 331-337. Purwokerto.

Benjamin, S and F. Spener. 2009. Conjugated linoleic acids as functional food: an insight into their health benefits. J. Nutr. Metabolism, 6: 36-48.

Chen, H., B. Yang., S. Gu., B. Zhang., Q. Xu., Q. Ye., Y. Song., Y.Q. Chen., H. Zhang and W. Chen. 2012. Purification and characteriza-tion of a linoleate isomerase from Lactobacillus plantarum ZS2058. African J. Biotech, 11 (20): 4579-4587.

de Carvalho, E.B.T., I.L.P. de Melo and F.J. Mancini. 2010. Chemical and physiological aspects of isomers of conjugated fatty acids. Ciencia ETecnologia De Alimentos, 30:295â€“307.

Devillard, E., F.M. McIntosh., D.Paillard., N.A. Thomas., K.J.Shingfieldand R.J. Wallace.2009. Differences between human subjects in the composition of the faecal bacterial community and faecal metabo-lism of linoleic acid. J. Micro-biology, 155: 513â€“520.

Gibson, G.R and M.B. Roberfroid.1995. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J. Nutr, 125: 1401-1412.

Gibson, G.R and R. Fuller. 2000.Aspects of in vitro and in vivo research approaches directed toward identifying probiotics and prebiotics for human use. J. Nutr, 130: 391-395.

Harwood, J.L. 1988. Fatty Acid Metabolism. Annu. Rev. Plant. Physiol. Plant. Mol. Biol, 39:101-138.

Howard, F.A.C and C. Henderson. 1999. Hydrogenation of polyunsaturated fatty acids by human colonic bacteria. Lett. Appl.Microbiol, 29: 193â€“196.

Margolles, A., B. Mayo and P. RuasMadiedo. 2009. Screen-ing, Identification, and Characterization of Lactobacillus and Bifidobacterium Strains, pp 4â€“24. In Y.K. Lee and S. Salminen (Eds.) Handbook of Probiotics and Prebiotics 2nd Edition. Hoboken: John Wiley & Sons Inc.

Martin, R., E. Jimenez., H. Heilig., L. Fernandez., M.L. Marin., E.G. Zoetendal and J.M. Rodriguez. 2009. Isolation of Bifidobacteria from breast milk and assessment of the bifidobacterial population by pcr-denaturing gradient gel electrophoresis and quantita-tive real-time PCR. Appl. Environ. Microbiol, 75 (4): 965-969.

McIntosh, F.M., R.J. Wallaceand E. Devillard. 2006. Variations in intermediates and products of linoleic acid biohydrogenation bytwo different human mixed colonic flora.J. Reprod. Nutr. Dev, 46(Suppl.1):S52.

Panghyova, E., D. Kacenova., S. Hajduskova., M. Matulova and E. Kiss. 2006. Influence of free linoleic acid on the fatty acids profileof fermentation by selected probiotic bacteria. J.Food and Nutr. Research, 45(4): 159-165.

Rainer, L and C.J. Heiss. 2004.Conjugated linoleic acid: Health implications and effects on body composition. J. Am.Diet Ass, 104 (6): 963-968.

Sharma, A.K., P. Mohan and B.B.Nayak. 2005. Probiotic: Making A Comeback. Depart-ment of Pharmacology Armed Forces Medical College: Pune.

Wells, J.M., O. Rossi., M. Meijerink and P. van Baarlen. 2010. Microbes and health sackler colloquium: epithelial cross-talk at the microbiota-mucosal interface. Proceedings of the National Academy of Sciences of the United States of America, 108 (1): 4607-4614.

Widodo., N.S. Anindita., T.T. Taufiq and T.D. Wahyuningsih. 2014. Evaluation of Two Lactobacillus Strains as Probiotics with Emphasis in Utilizing Prebiotic Inulin as Energy Source. International Research Journal of Microbiology (IRJM) (ISSN:2141-5463), Vol. 5(3): 33-40.

Widodo., N.S. Anindita., T.T. Taufiq dan T.D. Wahyuningsih. 2013. Identification and Selection of Human Origin Lactobacillus Strains as Probiotics With Capability in Synthesizing Conjugated Linoleic Acid (CLA). Third International Symposium on Probiotics and Prebiotics, Microbiome, GutBrain Axis In Health and Disease Mechanism, Function and Regulation. Jakarta.

Widodo., P.A. Harsita., N.S. Anindita., T.D. Wahyuningsihand A. Nurrochmad. 2015. Selection of Human-origin Lactobacillus strains as Probiotics with Capability in Synthesizing Conjugated Linoleic Acid and Alleviating Hyperglycemia in Rats (Rattus norvegicus) in vivo. The 6th International Seminar on Tropical Animal Production Integrated Approach in Developing Sustainable Tropical Animal Production. Yogyakarta. Indonesia.

Widodo., T. Taufiq., E. Aryati1., A. Kurniawati and W. Asmara. 2012. Human Origin Lactobacillus casei Isolated from Indonesian Infants Demonstrating Potential Characteristics as Probiotics in vitro. Indonesian Journal of Biotechnology, 17 (1): 79-89.

Widodo., N.S. Anindita., T.T. Taufiq and T.D. Wahyuningsih. 2012. Identification of Pediococcus Strains Isolated from Feces of Indonesian Infants With in vitro Capability to Consume Prebiotic Inulin and to Adhereon Mucus. Indonesian Journal of Biotechnology, 17 (2): 132-143.