The Detection of Genes Encoding Enzyme Fructosyltransferase The Exopolysaccharide (EPS) Produce Potential Probiotic Candidates For Breast Milk (ASI)

Afifah Nurul Falih; Nosa Septiana Anindita; Arif Bimantara; Annisa Khumaira

doi:10.31101/ijhst.v4i1.2526

Authors

Afifah Nurul Falih Universitas 'Aisyiyah Yogyakarta
Nosa Septiana Anindita Universitas 'Aisyiyah Yogyakarta, Indonesia
Arif Bimantara Universitas 'Aisyiyah Yogyakarta, Indonesia
Annisa Khumaira Universitas 'Aisyiyah Yogyakarta, Indonesia

DOI:

https://doi.org/10.31101/ijhst.v4i1.2526

Keywords:

breast milk, exopolysaccharide, fructosyltransferase, probiotics

Abstract

Bacteria is one of the microorganisms that we can easily find in nature. Bacteria can be classified into benefical bacteria and harmful bacteria. One of the bacteria classified as benefical bacteria is lactic acid bacteria (LAB). One of the bacteria included in LAB is probiotic bacteria.Â Probiotic bacteria can be isolated from several sources, one of which is breast milk. The functionality of probiotics is seen by the production of exopolysaccharides (EPS). EPS is produced through the expression of the fructosyltransferase (ftf) gene. The samples used in this study were 16 isolates of candidate probiotic bacteria from breast milk. The stages of this research included reculture of probiotic isolates, isolation of bacterial genomic DNA, amplification of isolates with 16s rRNA primers, amplification of isolates with primary lactic acid bacteria (LAB) and amplification of isolates with specific primers of ftf gene. The results showed that 10 of 16 probiotic bacterial isolates had the ftf gene indicated by the presence of DNA bands on the electrophoregram with a size 58 bp.Â

References

Anindita, N.S., Novalina, D., & Sholikhah, A. N. (2018) Isolasi Dan Identifikasi Bakteri Asam Laktat Asal Air Susu Ibu Sebagai Kandidat Probiotik Dalam Pangan Kesehatan. Lap Penelitian Dosen Pemula (PDP), Yogyakarta

Bilang, M., Tahir, M., & Haedar, D. (2018). Mempelajari Viabilitas Enkapsulasi Sel Probiotik (Lactobacillus plantarum dan Streptococcus thermophilus) pada es krim. Canrea Journal: Food Technology, Nutritions, and Culinary Journal, 1(1): 41-52.

Chun, J., & Goodfellow, M. (1995). A Phylogenetic analysis of The Genus Nocardia with 16S rRNA Gene Sequences. International Journal of Systematic and Evolutionary Microbiology, 45(2), 240-245.

Detha, A., Datta, F. U., Beribe, E., Foeh, N., & Ndaong, N. (2019). Karakteristik Bakteri Asam Laktat yang Diisolasi dari Susu Kuda Sumba. Jurnal Kajian Veteriner, 7(1), 85-92.

Halim, C., & Zubaidah, E. (2013). Studi Kemampuan Probiotik Isolat Bakteri Asam Laktat Penghasil Eksopolisakarida Tinggi Asal Sawi Asin (Brassica juncea). Jurnal Pangan dan Agroindustri, 1(1), 129-137.

Harutoshi, T. (2013). Exopolysaccharides of Lactic Acid Bacteria For Food and Colon Health Applications. Intech Open, London

Hermansyah., Sutami, N., & Miksusanti. (2018). Amplifikasi PCR Domain D1/D2 28S rDNA Menggunakan Primer ITS1 dan ITS4 Sampel DNA dari Candida tropicalis yang Diisolasi dengan Metode Pendinginan. Indonesian Journal of Pure and Applied Chemistry, 1(1), 1-9.

Jiang, M., Zhang, F., Wan, C., Xiong, X., Shah, N., Wei, H., & Tao, X. (2016). Evalutation of Probiotic Properties of Lactobacillus Plantarum WLPL04 Isolated From Human Breast Milk. Journal Dairy Science, 99(3), 1736-1746

Karamoy, E., & Kepel, B. (2017). Isolasi dan Identifikasi Bakteri Resisten Merkuri Melalui Analisis Gen 16S Rrna Pada Urin Pasien Dengan Tumpatan Amalgam. Jurnal eBiomedik, 5(2), 1-8.

Malik, A., Hermawati, A., Hestiningtyas, M., Soemiati, A & Radji, M. (2010). Isolasi dan Skrining Molekuler Bakteri Asam Laktat Pembawa Gen Glukansukrase dari Makanan dan Minuman Mengandung Gula. Makara Sains, 14(1), 63-68.

Malik, A., Radji, M., Kralj, S & Dijkhuizen, L. (2009). Screening of Lactic Acid Bacteria From Indonesia Reveals Glucansucrase And Fructansucrase Genes In Two Different Weissella Confusa Strains From Soya. FEMS microbiology letters, 300(1), 131-138.

Mardalena. (2016). Fase Pertumbuhan Isolat Bakteri Asam Laktat (BAL) Tempoyak Asal Jambi yang Disimpan Pada Suhu Kamar. Jurnal Sain Peternakan Indonesia (JSPI), 11(1), 58-66.

Muhammad, B., Fadli, Z., & Risandiansyah, R. (2020). Perbandingan Isolasi DNA Bakteri Escherichia coli dengan Metode Heat Treatment Dan Filter Based Kit Berdasarkan Nilai Limit of Detection dan Limit of Quantification. Jurnal Kedokteran Komunitas, 8(2), 66-73.

Noor, S., Pramono, H., & Aziz, S. (2014). Deteksi Keragaman Spesies Bakteri Metanogen Rumen Sapi Menggunakan Kloning Gen 16S rRNA dan Sekuensing. Scripta Biologica, 1(4), 1-8.

Nudyanto, A., & Zubaidah, E. (2015). Isolasi Bakteti Asam Laktat Penghasil Eksopolisakarida dari Kimchi. Jurnal Pangan dan Agroindustri, 3(2), 743-748.

Nurhasanah., Fuâ€™adah, I., Satria, H., & Yuwono, S. (2020). Analisis Eksopolisakarida dari Bakteri Asam Laktat Hasil Fermentasi Kefir Kolostrum. Analit: Analytical and Enviromental Chemistry, 5(01), 65-73.

Nurwati, L., & Nawfa, R. (2015). Identifikasi Spesies Isolat Bakteri Galur D dengan Metode Analisa Sekuen Fragmen Gen 16S rDNA. Jurnal Sains dan Seni ITS, 4(2), 126-129

Ortiz, C., Matel, H., & Nellas, R. (2019). In Silico Insights on Enhancing Thermostability And Activity of a Plant Fructosyltransferase from Pachysandra terminalis Via Introduction of Disulfide Bridges. Journal of Molecular Graphics and Modelling, 89, 250-260

Reis, N. A., Saraiva, M. A. F., Duarte, E. A. A., de Carvalho, E. A., Vieira, B. B., & Evangelistaâ€Barreto, N. S. (2016). Probiotic Properties of Lactic Acid Bacteria Isolated from Human Milk. Journal of applied microbiology, 121(3), 811-820.

Rinanda, T. (2011). Analisis Sekuensing 16S rRNA di Bidang Mikrobiologi. Jurnal Kedokteran Syiah Kuala, 11(3), 172-177.

Safitri, N., Sunarti,, T & Meryandini, A. (2017). Formula Media Pertumbuhan Bakteri Asam Laktat Pediococcus pentosaceus Menggunakan Substrat Whey Tahu. Jurnal Sumberdaya Hayati, 2(2), 31-38.

Serrano-Nino, J., Solis-Pacheco, J., Gutierrez-Padilla, J., Cobian-Garcia, A., Cavazos-Garduno, A., Gonzalez Reynoso, O., & Aguilar-Uscanga, B.(2016). Isolation and Identification of Lactic Acid Bacteria from Human Milk with Potential Probiotic Role. Journal Food and Nutrition Research, 4(3), 170-177.

Seveline, S.(2018). Kajian Pustaka Teknik Pengeringan Semprot (Spray Drying) untuk Pengawetan dan Produksi Probiotik. Jurnal Agroindustri Halal, 3(1), 80-86.

Shehata, M. G., El Sohaimy, S. A., El-Sahn, M. A., & Youssef, M.(2016). Screening of Isolated Potential Probiotic Lactic Acid Bacteria for Cholesterol Lowering Property and Bile Salt Hydrolase Activity. Annals of Agricultural Sciences, 61(1),65-75.

Singh, V., Chaudhary, D., & Mani, I.(2012). Molecular Characterization and Modeling of Secondary Structure of 16S rRNA from Aeromonas veronii. International Journal Applied Biology and Pharmaceutical Technology, 3(1), 253-260.

Sunaryanto, R., Martius, E., & Marwoto, B. (2014). Uji Kemampuan Lactobacillus casei sebagai Agensia Probiotik. Jurnal Bioteknologi & Biosains Indonesia, 1(1), 9-14

Surbakti, F. H., & Hasanah, U. (2019). Identifikasi dan Karakterisasi Bakteri Asam Laktat pada Ketimun (Cucumis sativus L.) sebagai Agensi Probiotik.Jurnal Teknologi Pangan dan Kesehatan, 1(1), 31-37

Susilawati, L., & Purnomo, E. (2016). Viabilitas Sel Bakteri dengan Cryoprotectant Agents Berbeda (Sebagai Acuan dalam Preservasi Culture Collection di Laboratorium Mikrobiologi). Biogenesis: Jurnal Ilmiah Biologi, 4(1), 34-40.

Yang, Z., Xue, Y., Rao, S., Zhang, M., Gao, L., Yin, Y., Chen, D., Zhou,X., & Jiao, X. (2017). Isolation of Probiotic Piliated Lactobacillus rhamnosus Strains from Human Fecal Microbiota Using SpaA Antiserum-Based Colony Immunoblotting. Journal Microbiology Biotechnology, 27(11), 1971-1982

Zannini, E., Waters, D., Coffey, A., & Arendt, E. 2016. Production, Properties, And Industrial Food Application Of Lactic Acid Bacteria-Derived Exopolysaccharides. Applied Microbiology And Biotechnology, 100(3), 1121-1135.

Zhang, D., & Zhang, S. 2018. Isolation and Characterization of New Probiotic Strains from Chinese Babies. Journal Clin Gastroenteol, 52(1), 27-34.